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PCR Method Comparison

 

Traditional Methods
Molecular Methods
Sample
Turnaround
Time
1-2 weeks (culturable samples) 1-2 days on any sample
Data Quality
Can be variable based upon analyst and experience Instrument-based data
Aspergillus /
Penicillium
Differentiation
Requires 1-2 weeks Requires 1-2 days
Detection System
Microscopical detection utilizing color, size and morphology of mold Highly sensitive instrument-based detection relying on DNA sequence
Sampling
Times
Typically short term sampling Able to take long term samples
Background
Interference
Potential
Dust particulate can cause interference on Air-O-Cells, Burkhards, Allergenco, tape lifts, or bulk non-viable direct exams Particulate in the sample not an interference as PCR will not amplify non-targeted material; Clean up of samples available